Controlled release of nerve growth factor from heparin-conjugated fibrin gel within the nerve growth factor-delivering implant
Identifieur interne : 000903 ( Main/Exploration ); précédent : 000902; suivant : 000904Controlled release of nerve growth factor from heparin-conjugated fibrin gel within the nerve growth factor-delivering implant
Auteurs : Jin-Yong Lee [Corée du Sud] ; Soung-Min Kim [Corée du Sud] ; Myung-Jin Kim [Corée du Sud] ; Jong-Ho Lee [Corée du Sud]Source :
- Journal of the Korean Association of Oral and Maxillofacial Surgeons [ 2234-7550 ] ; 2014.
Abstract
Although nerve growth factor (NGF) could promote the functional regeneration of an injured peripheral nerve, it is very difficult for NGF to sustain the therapeutic dose in the defect due to its short half-life. In this study, we loaded the NGF-bound heparin-conjugated fibrin (HCF) gel in the NGF-delivering implants and analyzed the time-dependent release of NGF and its bioactivity to evaluate the clinical effectiveness.
NGF solution was made of 1.0 mg of NGF and 1.0 mL of phosphate buffered saline (PBS). Experimental group A consisted of three implants, in which 0.25 µL of NGF solution, 0.75 µL of HCF, 1.0 µL of fibrinogen and 2.0 µL of thrombin was injected via apex hole with micropipette and gelated, were put into the centrifuge tube. Three implants of experimental group B were prepared with the mixture of 0.5 µL of NGF solution, 0.5 µL HCF, 1.0 µL of fibrinogen and 2.0 µL of thrombin. These six centrifuge tubes were filled with 1.0 mL of PBS and stirred in the water-filled beaker at 50 rpm. At 1, 3, 5, 7, 10, and 14 days, 1.0 mL of solution in each tubes was collected and preserved at -20℃ with adding same amount of fresh PBS. Enzyme-linked immunosorbent assay (ELISA) was done to determine
The average concentration of released NGF in the group A and B increased for the first 5 days and then gradually decreased. Almost all of NGF was released during 10 days. Released NGF from two groups could promote neural differentiation and neurite outgrowth of PC12 cells and these bioactivity was maintained over 14 days.
Controlled release system using NGF-HCF gel via NGF-delivering implant could be an another vehicle of delivering NGF to promote the nerve regeneration of dental implant related nerve damage.
Url:
DOI: 10.5125/jkaoms.2014.40.1.3
PubMed: 24627836
PubMed Central: 3949490
Affiliations:
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<author><name sortKey="Kim, Soung Min" sort="Kim, Soung Min" uniqKey="Kim S" first="Soung-Min" last="Kim">Soung-Min Kim</name>
<affiliation wicri:level="4"><nlm:aff id="A2">Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University, Seoul, Korea.</nlm:aff>
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<author><name sortKey="Lee, Jong Ho" sort="Lee, Jong Ho" uniqKey="Lee J" first="Jong-Ho" last="Lee">Jong-Ho Lee</name>
<affiliation wicri:level="4"><nlm:aff id="A2">Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University, Seoul, Korea.</nlm:aff>
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<series><title level="j">Journal of the Korean Association of Oral and Maxillofacial Surgeons</title>
<idno type="ISSN">2234-7550</idno>
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<front><div type="abstract" xml:lang="en"><sec><title>Objectives</title>
<p>Although nerve growth factor (NGF) could promote the functional regeneration of an injured peripheral nerve, it is very difficult for NGF to sustain the therapeutic dose in the defect due to its short half-life. In this study, we loaded the NGF-bound heparin-conjugated fibrin (HCF) gel in the NGF-delivering implants and analyzed the time-dependent release of NGF and its bioactivity to evaluate the clinical effectiveness.</p>
</sec>
<sec><title>Materials and Methods</title>
<p>NGF solution was made of 1.0 mg of NGF and 1.0 mL of phosphate buffered saline (PBS). Experimental group A consisted of three implants, in which 0.25 µL of NGF solution, 0.75 µL of HCF, 1.0 µL of fibrinogen and 2.0 µL of thrombin was injected via apex hole with micropipette and gelated, were put into the centrifuge tube. Three implants of experimental group B were prepared with the mixture of 0.5 µL of NGF solution, 0.5 µL HCF, 1.0 µL of fibrinogen and 2.0 µL of thrombin. These six centrifuge tubes were filled with 1.0 mL of PBS and stirred in the water-filled beaker at 50 rpm. At 1, 3, 5, 7, 10, and 14 days, 1.0 mL of solution in each tubes was collected and preserved at -20℃ with adding same amount of fresh PBS. Enzyme-linked immunosorbent assay (ELISA) was done to determine <italic>in vitro</italic>
release profile of NGF and its bioactivity was evaluated with neural differentiation of pheochromocytoma (PC12) cells.</p>
</sec>
<sec><title>Results</title>
<p>The average concentration of released NGF in the group A and B increased for the first 5 days and then gradually decreased. Almost all of NGF was released during 10 days. Released NGF from two groups could promote neural differentiation and neurite outgrowth of PC12 cells and these bioactivity was maintained over 14 days.</p>
</sec>
<sec><title>Conclusion</title>
<p>Controlled release system using NGF-HCF gel via NGF-delivering implant could be an another vehicle of delivering NGF to promote the nerve regeneration of dental implant related nerve damage.</p>
</sec>
</div>
</front>
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